Journal: Frontiers in Oncology

Article Title: Overexpression of lncRNA SLC16A1-AS1 Suppresses the Growth and Metastasis of Breast Cancer via the miR-552-5p/WIF1 Signaling Pathway

doi: 10.3389/fonc.2022.712475

Figure Lengend Snippet: Expression of SLC16A1-AS1 is significantly downregulated in breast cancer (BC) cells and tissues and related to the prognosis of BC patients. (A) Bioinformatics analysis found downregulated SLC16A1-AS1 and MEG3 but upregulated HOTAIR expressions in tumor (T) tissues versus normal (N) tissues of BC (BRCA) patients by investigating the TCGA datasets using the online tool GEPIA. (B) SLC16A1-AS1 and MEG3 expressions were meaningfully downregulated, while HOTAIR expression was meaningfully upregulated in BC tissues versus paracancerous tissues from 80 BC patients analyzed by the qRT-PCR assay. (C) SLC16A1-AS1 expression was meaningfully lower in human BC (MCF7, MDA-MB-231, MB453, and BT474) than in human normal mammary gland epithelial cells (MCF 10A) analyzed with qRT-PCR, and the difference was most significant in MCF7 and MDA-MB-231 cells. (D, E) Analyzed by the Kaplan–Meier method among the 80 BC patients, lower SLC16A1-AS1 expression indicated a poor prognosis both in overall survival (D) and disease-free survival (E) . * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: A human normal mammary gland epithelial (MCF 10A) and four human BC (MCF7, MDA-MB-231, MB453, and BT474) cell lines were provided by the American Type Culture Collection (Manassas, USA) and maintained in DMEM containing 100 μg/ml streptomycin, 100 U/ml penicillin, and 10% FBS in a 37°C incubator with 5% CO 2 .

Techniques: Expressing, Quantitative RT-PCR

Journal: Frontiers in Oncology

Article Title: Overexpression of lncRNA SLC16A1-AS1 Suppresses the Growth and Metastasis of Breast Cancer via the miR-552-5p/WIF1 Signaling Pathway

doi: 10.3389/fonc.2022.712475

Figure Lengend Snippet: SLC16A1-AS1 increases WIF1 expression via sponging miR-552-5p. (A) Illustration of the prospective binding sites of miR-552-5p in WIF1 3′-UTR predicted with miRanda ( http://www.microrna.org/ ). (B) mRNA expression of WIF1 was significantly lower in BC cells (MDA-MB-231 and MCF7) than in normal mammary gland epithelial cells (MCF 10A) analyzed by qRT-PCR. (C) Dual-luciferase activities in MDA-MB-231 and MCF7 cells with negative control (NC), wild type (WT), or mutated (mut) reporter of WIF1 3′-UTR with miR-552-5p or without (NC) miR-552-5p mimics. (D) mRNA and protein expressions of WIF1 in MCF7 and MDA-MB-231 cells after miR-552-5p was silenced (miR-552-5p inhibitor) or overexpressed (miR-552-5p mimics). (E) mRNA (left panel) and protein expressions (middle and right panels) of WIF1 in SLC16A1-AS1 overexpressed in MDA-MB-231 and MCF7 cells with or without co-transfecting miR-552-5p. (F) Spearman correlation assay of the correlation between WIF1 and SLC16A1-AS1 or miR-552-5p in BC tissues from 80 BC patients (the same specimens used in Figure 1A ). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: A human normal mammary gland epithelial (MCF 10A) and four human BC (MCF7, MDA-MB-231, MB453, and BT474) cell lines were provided by the American Type Culture Collection (Manassas, USA) and maintained in DMEM containing 100 μg/ml streptomycin, 100 U/ml penicillin, and 10% FBS in a 37°C incubator with 5% CO 2 .

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Luciferase, Negative Control, Two-Photon Excitation Fluorescence Cross-Correlation Assay

Journal: Scientific Reports

Article Title: Association between Galectin-3 levels within central and peripheral venous blood, and adverse left ventricular remodelling after first acute myocardial infarction

doi: 10.1038/s41598-019-49511-4

Figure Lengend Snippet: Echocardiography parameters used to diagnose systolic and diastolic left ventricular dysfunction on day 1, 30 and 180, in patients with and without left ventricular remodelling, six months after acute myocardial infarction.

Article Snippet: Day-30 Galectin-3 also showed positive correlations with echocardiography parameters indicative of diastolic and systolic dysfunction.

Techniques:

Journal: Scientific Reports

Article Title: Association between Galectin-3 levels within central and peripheral venous blood, and adverse left ventricular remodelling after first acute myocardial infarction

doi: 10.1038/s41598-019-49511-4

Figure Lengend Snippet: Correlation between plasma Galectin-3 levels and echocardiography parameters determined 6 months after acute myocardial infarction.

Article Snippet: Day-30 Galectin-3 also showed positive correlations with echocardiography parameters indicative of diastolic and systolic dysfunction.

Techniques: Clinical Proteomics